Forskolin stimulates α-amylase release in rat parotid tissue
نویسندگان
چکیده
منابع مشابه
Effect of Cytochalasin B and Colchicine on Α-amylase Release from Rat Parotid Tissue Slices
We have recently reported (3) that cytochalasin B, which disrupts microfilaments, and colchicine, which disrupts microtubules, interfered with the release of a-amylase from rat parotid tissue slices . Our observations on the effect of cytochalasin B on a-amylase release have been confirmed by Rifkin et al . (14) . Inhibitory actions of cytochalasin B or colchicine in several other systems have ...
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1. A method is described for the isolation of rat parotid acinar cells by controlled digestion of the gland with trypsin followed by collagenase. As judged by Trypan Blue exclusion, electron microscopy, water, electrolyte and ATP concentrations and release of amylase and lactate dehydrogenase, the cells are morphologically and functionally intact. 2. A method was developed for perifusion of aci...
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At concentrations greater than 0.01 microM, thapsigargin (ThG) dose-dependently caused an increase in cytosolic free Ca2+ concentration ([Ca2+]i) in rat parotid acinar cells, as measured by the fluorescent Ca(2+)-indicator fura-2. In the absence of extracellular Ca2+, a transient increase in [Ca2+]i by ThG was observed, and subsequent addition of carbachol (CCh) did not produce a further [Ca2+]...
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In parotid acinar cells, activation of beta-adrenergic receptors provokes exocytotic amylase release via the accumulation of intracellular cAMP. Cellular redox status plays a pivotal role in the regulation of various cellular functions. Cellular redox imbalance caused by the oxidation of cellular antioxidants, as a result of oxidative stress, induces significant biological damages. In this stud...
متن کاملThe small GTPase Rab27B regulates amylase release from rat parotid acinar cells.
Small GTPase Rab is a large family of putative membrane trafficking proteins, and each member is thought to regulate a specific type(s) of membrane trafficking. However, little is known about the involvement of Rab protein(s) in secretory granule exocytosis in exocrine cells or the molecular mechanism underlying this process. We show that Rab27B, a closely related isoform of Rab27A that regulat...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1983
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)60248-9